Augusto Simões-Barbosa; Nancy R. Sturm; Antônio R.L Teixeira
Laboratório Multidisciplinar de Pesquisa em Doença de Chagas. Faculdade de Ciências da Saúde, Universidade de Brasília, Brasília, DF e Department of Microbiology and Immunology, UCLA School of Medicine, Los Angeles, CA.
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REFERENCES
1. Cruz A, Beverly SM. Gene replacement in parasite protozoa. Nature 348:171-173,1990. [ Links ]
2. Cruz A, Coburn CM, Beverly SM. Double targeted gene replacement for creating null mutants. Proceedings National Academy Sciences 88:7170- 7174,1991. [ Links ]
3. Kelly JM, Tomas AM. An approach to functional complementation by introduction of large DNA fragments into Trypanosoma cruzi and Leishmania donovani using a shuttle vector. Molecular Biochemical Parasitology 65:51-62, 1994. [ Links ]
4. Kelly JM, Ward HM, Miles MA, Kendall G. A shuttle vector which facilitates the expression of transfected genes in Trypanosoma cruzi and Leishmania. Nucleic Acids Research 20:3963- 3969. [ Links ]
5. LeBowitz JH, Coburn CM, McMahon Prat D, Beverly SM. Development of a stable Leishmaniaexpression vector and application to the study of parasite surface antigen genes. Proceedings National Academy Sciences 87:9736-9740,1990. [ Links ]
6. Pasion SG, Brown GW. Brown LM, Ray DS. Periodic expression of nuclear and mitochondrial DNA replication genes during the trypanosomatid cell cycle. Journal Cell Sciences 107:3515-3520, 1994. [ Links ]
7. Vainstein MH, Alves SA, de Lima BD, Aragao JL, Rech EL. Stable transfection in a flagellate trypanosomatid by microparticle bombardment. Nucleic Acids Research 22:3263-3264, 1994. [ Links ]
Recebido para publicação em 28/02/96.
Laboratório Multidisciplinar de Pesquisa em Doença de Chagas. Faculdade de Ciências da Saúde, Universidade de Brasília, Brasília, DF e Department of Microbiology and Immunology, UCLA School of Medicine, Los Angeles, CA.
Adress to correspondence:
Dr. Antonio R.L. Teixeira,
Laboratório Multidisciplinar de Pesquisa em Doença de Chagas,
Faculdade de Ciências da Saúde,
Universidade de Brasília.
Caixa Postal 04-685,70919-970
Brasília, DF, Brasil.
Fax: (061) 273-4645.
