Jesila Pinto M. Marretto; Sonia G. Andrade
ABSTRACT
To investigate the influence of chemotherapy on the biochemical beha vior of Trypanosoma cruzi strains, three groups of mice were infected with one of three strains of T. cruzi of different biological and isoenzymic patterns (Peruvian, 21 SF and Colombian strains). Each group was subdivided into subgroups: 1 – treated with nifurtimox; 2 – treated with benznidazole and 3 – untreated infected controls. At the end of treatment, that lasted for 90 days, xenodiagnosis, sub inoculation of blood into new born mice and haemoculture were performed as tests of cure. From the positive tests, 22 samples of T. cruzi were isolated from all subgroups. Electrophoretic analysis of the isoenzymes PGM, GP1, ALAT and AS AT failed to show any difference between parasite strains isolated from treated and untreated mice, which indicates that no detectable clonal selection or parasite genetic markers alterations concerning the isoenzymes analysed have been determined by treatment with drugs of recognized antiparasitic effect, suggesting stability of the phenotypic characteristics of the three biological types of T. cruzi strains.
Keywords: Trypanosoma cruzi strains. Isoenzymes. Chemotherapy.
RESUMO
Com o objetivo de investigar a influência da quimioterapia no padrão bioquímico de diferentes cepas do Trypanosoma cruzi, três grupos de camundongos foram infectados respectivamente com as cepas Peruana, 21 SF e Colombiana, que correspondem a diferentes padrões biológicos e isoenzimáticos. Cada grupo foi subdividido em subgrupos: 1 – tratados com nifurtimox; 2 – tratados com benzonidazol; 3- controles infectados não tratados. Ao final do tratamento que durou 90 dias, os animais foram submetidos a testes parasitológicos de cura: xenodiagnóstico, subinoculação do sangue em camundongos recém-nascidos e hemocultura em meio Warren. A partir da positivação destes testes, foram isoladas 22 amostras do T. cruzi dos três subgrupos. A análise eletroforética dos extratos enzimáticos obtidos após cultura para as enzimas PGM, GPI, ALAT e ASAT demonstrou identidade dos padrães enzimáticos entre os parasitos de uma mesma cepa isolados de animais tratados ou não tratados. Conclui-se que não houve seleção clonal ou alterações genéticas dos parasitos, detectáveis pelas isoenzimas examinadas, dependentes da ação de drogas de reconhecida ação antiparasitária. Isto sugere uma estabilidade dos caracteres fenotípicos dos três tipos biológicos de cepas do T. cruzi.
Palavras-chave: Cepas do Trypanosoma cruzi. Isoenzimas. Quimioterapia.
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Adress to correspondence:
Dra Sonia G. Andrade.
Centro de Pesquisas Gonçalo Moniz/FIOCRUZ/UFBA.
R. Valdemar Falcão 121 Brotas,
40295-001
Salvador, BA, Brasil.
Recebido para publicação em 25/03/94.
Centro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz, Universidade Federal da Bahia, Salvador, BA.
