Home » Volumes » Volume 24 April/June 1991 » Two competitive enzyme immunoassays for the detection of IgG class antibodies to hepatitis a antigen

Two competitive enzyme immunoassays for the detection of IgG class antibodies to hepatitis a antigen

Claudia Lamarca Vitral; Ana Maria Coimbra Gaspar; Clara Fumiko Tachibana Yoshida

DOI: 10.1590/S0037-86821991000200003


Two competitive enzyme immunoassays (EIA) techniques were developed: in the first (COMP-1), test sera were added together with HAV antigen on anti-HAV IgG-coated wells followed by an anti-HA VHRP conjugate; in the second (COMP-2), test sera and anti-HA VHRP conjugate competed for HAV epitopes previously adsorbed to anti-HA V IgG-coated wells. Both procedures used tetramethylbenzidine (TMB) as a substrate. Both competitive tests were shown to be reproducible and suitable for routine diagnosis and research purposes.

Keywords: Enzyme immunoassay. Anti-HAV. Hepatitis A.


Foram desenvolvidos dois ensaios imunoenzimáticos (EIA) competitivos: no primeiro (COMP-1) colocou-se numa placa sensibilizada com anti-HAVIgG as amostras teste juntamente como antígeno HA Vea seguir o conjugado anti-HA VHRP; no segundo (COMP-2), as amostras teste e o conjugado anti-HAV HRP competem pelos epitopos do antígeno HAV previamente absorvido na placa sensibilizada do anti-HAV IgG. O substrato utilizado foi tetrametilbenzidina (TMB). Ambas as técnicas mostraram ser produtíveis e aplicáveis para fins de diagnóstico e pesquisa.

Palavras-chave: Enzyme immmunoassay. Anti-HAV. Hepatitis A.



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 Adress to correspondence:
Dra. Claudia Lamarca Vitral.
Depto. of Virology/FIOCRUZ.
Av. Brasil 4365
Rio de Janeiro, RJ, Brazil.



Recebido para publicação em 16/01/91.



Departamento of Virology, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brazil.