Home » Volumes » Volume 19 Octuber/December 1986 » Comportamento da cepa LE de Schistosoma mansoni, após passagem em hospedeiro humano infectado acidentalmente

Comportamento da cepa LE de Schistosoma mansoni, após passagem em hospedeiro humano infectado acidentalmente

Neusa Araújo; Cecília Pereira de Souza; Emmanuei Pinto Dias; Naftale Katz

DOI: 10.1590/S0037-86821986000400003


RESUMO

Uma auxiliar de laboratório infectou-se acidentalmente, com cercárias de Schistosoma mansoni, cepa LE, mantida rotineiramente em nossos laboratórios. Decorridos 5 meses, o exame parasitológico de fezes revelou 108 ovos/g . A pacientefoi tratada com oxamniquine, porém a infecção continuou ativa (6 ovos/g). Foi então obtido o isolado SSF mantido no modelo Biomphalaria glabrata – camundongo albino. Os resultados obtidos no estudo comparativo, entre o isolado SSF e a cepa LE, que lhe deu origem, mostraram que a duração do período pré-patente e o índice de infectividade em camundongos, bem como a resposta aos agentes esquistossomicidas (hycanthone, oxamniquine epraziquantel) não apresentaram diferenças estatisticamente significativas. Por outro lado, o número de miracídios obtidos dos intestinos e fígados dos camundongos infectados foi o dobro com a cepa LE, quando comparados com aquele do isolado SSF. Também a variação do peso dos animais foi bastante diferente. Concluiu-se que apenas uma passagem pelo hospedeiro humano não mudou substancialmente as características da cepa estudada.

Palavras-chave: Schistosoma mansoni Cepa. Estudo experimental comparativo.


ABSTRACT

A laboratory technician was accidentally infected with the LE strain of Schistosoma mansoni cercariae maintained in our laboratories. After 5 months infection parasitological examination revealed 108 eggs/g faeces. Althrough treated with oxamniquine, the infection still persisted (6 eggs/g of faeces). An isolate (SSF) was obtained from that person and maintained in Biomphalaria glabrata – Swiss mouse by alternate passage. The experimental comparative results between the newly obtained SSF isolate and the original LE strain showed that the differences in pre-patent period, the mice infectivity level and the schistosomicidal response to hycanthone, oxamniquine and praziquantel was not statistically significant. On the other hand the number of miracidia recovered from intestines and livers of equally infected mice was twice as miich in the LE strain than in the SSF isolate. Furthermore, among the LE and SSF infected mice and the non infected control weight gains were different. While the LE infected mice lost weight since the beginning of the infection, the SSF ones initially gained and then lost weight. The conclusion is that only one passage into the human host did not substantially alter the studied strain characteristics.

Keywords: Schistosoma mansoni. Strain Comparative experimental study.


 

 

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Recebido para publicação em 25/11/85.

 

 

Do Centro de Pesquisas “René Rachou” – Fundação Oswaldo Cruz, Ministério da Saúde, C.P. 1743, 30000 Belo Horizonte, MG, Brasil.
Trabalho subvencionado parcialmente pelo Conselho de Desenvolvimento Cientifico e Tecnológico (CNPq) e Financiadora de Estudos e Pesquisas (FINEP).