Amélia Dulce Vilela de CarvalhoI; Roberto Junqueira de AlvarengaII; Alan Lane de MeloIII
IDepartamento de Morfologia do Instituto de Ciências Biológicas da Universidade Federal de Minas Gerais, Belo Horizonte, Brasil IIDepartamento de Patologia Geral, ICB/UFMG IIIDepartamento de Parasitologia, ICB/UFMG
RESUMO
Exemplares de Biomphalaria glabrataforam infectados com miracídios obtidos de ovos de Schistosoma mansoni, encontrados em fezes de indivíduos de 7 a 18 anos, da região de Lagoa da Pampulha, Belo Horizonte, MG. Os pacientes de fase aguda se infectaram em uma primeira visita ao foco. Os da fase crônica eram moradores próximos aos focos. Para cada caso clínico, isolou-se a respectiva amostra do parasita. Foram infectados pela cauda 55 camundongos fêmeas com 70 ± 10 cercárias. Cortes histológicos de fígados, corados por HE, tricrômico de Gomori, impregnação metálica pela prata, e PAS foram observados à microscopia óptica. Não houve diferenças estatísticas em relação às médias das mensurações dos diâmetros dos granulomas referentes às amostras e datas de sacrifícios. Os granulomas apresentaram fase exsudativa do tipo Ha (reação de inflamação mista) e IIIa (granuloma com células epitelióides). Com amostras de pacientes em fase aguda o padrão predominante foi a Ha na 7a semana. Na 10.a semana predominaram granulomas do tipo IIIa. Nas amostras de pacientes em fase crônica, verificou-se uma mescla de granulomas do tipo Ha e IIIa na 7a semana. Na 10a semana predominou o tipo IIIa. Alguns aspectos histopatológicos de fígados foram descritos e comparados com aqueles existentes na literatura.
Palavras-chave: Schistosoma mansoni. Histopatologia. Granuloma.
ABSTRACT
Specimens o/Biomphalaria glabrata were infected with miracidia obtained from eggs of Schistosoma mansoni found in the feces of patients aged 7 to 18 years old, from Lagoa da Pampulha area, Belo Horizonte, Minas Gerais, tírazil. The patients in the acnte phase were infected on their first visit to the focus. The patients with chronic phase disease were living around the focus. Egg samples were isolate from each clinical case. Fifty five female miee were infected by their tails with 70 ± 10 cercaria Seclions of lirer were stained bv HE, Gomori trichrome, silver impregnation, and PAS, and examined by optical microscopy. No statistical difference was observed between the average diameter of the granulomas and the sample and date of sacrifice of the mice (7th and 10th weeks). The granulomas were in the exudative phase; type IIa (mixed inflammatory reactions) and type IIIa (granulomas with epithelioid cells). At the 7th week samples of the patients in acute phase showed granulomas type IIa. Type IIIa were predominam at the 10th‘ week. At the 7th week, in samples from chronic phase patients, a mixture of type lia and IIIa granulomas was observed.
Some histopathologic aspects of the liver are described and compared with those already found in the literature.
Keywords: Schistosoma mansoni. Histopathology. Granuloma.
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Endereço para correspondência:
Dra. Amélia Dulce Vilela de Carvalho
Rua Santiago 118, ap. 302
Sion
30310
Belo Horizonte, MG, Brasil.
Recebido para publicação em 18/10/84.
Trabalho realizado com o auxilio do Conselho Nacional de Desenvolvimento Cientifico e Tecnológico – CNPq e Universidade Federal de Minas Gerais, Brasil.
