F. SantoroI; M. SadigurskyII; I. SherlockII; D. AfchainI; A. CapronI
ICentre dimmunologie et de Biologie Parasitaire (Unité Mixte INSERM U 167 - CNRS 624), Institut Pasteur, 1, rue du Prof. A. Calmette, 59019 LILLE Cédex, France IICentro de Pesquisas Gonçalo Moniz (FIOCRUZ/UFBa), 40000 Salvador, Bahia, Brazil
ABSTRACT
A competitive antibody enzyme immunoassay, using a monoclonal antibody against the species-specific Trypanosoma crnzi antigen 5, was used to investigate the presence of anti-component 5 antibodies in sera of opossums, dogs, rabbits and rats infected with this parasite. The sera from 51 Venezuelan patients with Chagasdisease were also tested. About 90% of the infected subjects showed significant levels of anti-component 5 antibodies. Nevertheless, these antibodies were not detected in the sera of dogs, rats and opossums infected with T. cruzl Some sera from infected rabbits presented significant results but close to the limit ofpositivity ofthe test. These findings suggest that the immune response in animals naturally or experimentally infected with T. cruzi is different from that observed in human Chagasdisease.
Keywords: Trypanosoma cruzi. Antibodies. Immunoassay.
RESUMO
Foi usado um teste imunoenzimático competitivo para investigar a presença de anticorpos anticomponente 5 nos soros de sariguês, cães, coelhos e ratos infectados com o Trypanosoma cruzi. Neste teste, foi utilizado um anticorpo monoclonal contra o antígeno 5 que é específico do T. cruzi. Também foram testados os soros de 51 pacientes venezuelanos com doença de Chagas.
Apesar desses anticorpos não serem detectados nos soros de cães, ratos e sariguês infectados com o T. cruzi, alguns soros de coelhos infectados apresentaram resultados positivos porém em níveis próximos aos do limite de positividade do teste. Esses achados sugerem que a resposta imune em animais naturalmente ou experimentalmente infectados com o T. cruzi é diferente daquela que é observada na doença de Chagas humana.
Palavras-chave: Trypanosoma cruzi. Anticorpos. Imuno-ensaio.
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Adress to correspondence:
Dr. Reinaldo Martinelli.
FACP/ Laboratório de Nefrologia – 1117
Hospital Universitário
Prof. Edgard Santos.
Rua João das Botas s/n, Canela
40110-160
Salvador, BA, Brasil.
Recebido para publicação em 19/11/92.
Department of Medicine, Federal University of Bahia Medical School, Salvador BA Brazil.
This study was supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico – CNPq, Brazil (Grant n° 407670/85CL).
