Maria Orleide Pires Borba
Mestre em Medicina Tropical — Profa. Colaboradora da Disciplina de Medicina Tropical da Universidade Federal de Pernambuco
RESUMO
O estudo das formas isodinâmicas da fosfatase alcalina sérica em pacientes esquistossomáticos, revelou modificações de suas propriedades físico-químicas na fase hépato intestinal e hepatosplênica compensada e descompensada, comparando-se com as da fosfatase alcalina sérica de indivíduos considerados como normais. Por filtração molecular em Sephadex G—200 foi possível separarar três (3) formas moleculares distintas de fosfatase alcalina sérica na esquistossomose mansônica hepatosplênica compensada e descompensada, duas das quais se apresentaram como termorresistentes, diferindo das equivalentes formas isodinâmicas de soro normal, que são termolábeis. Na fase hépato intestinal da esquistossomose mansônica, empregando a mesma metodologia, foi detectada uma única forma da fosfatase alcalina, termolábil, que parece corresponder à primeira forma isodinâmica do soro normal. Foi também realizado um estudo das diferentes formas moleculares de fosfatase alcalina humana de: fígado, intestino delgado, osso e placenta provenientes de tecidos considerados normais, bem como do extrato de Schistosoma mansoni.
ABSTRACT
The study of the multipls formas of serum alkaline phosphatase in patients with schistosomiasis (hepato intestinal compensated and decompesated hepatosplenic) revealed modifications in their physico-chemical properties. Molecular filtration with a Sephadex G—200 colun separated three distrinct molecular forms of serum alkaline phosphatase in patients with hepatosplenic schistosomiasis mansoni. Two of these forms presented as thermoresistant, contrasting with the equivalente multpls forms in normal serum which are termolabile, In the hepatointestinal phase of schistosomiasis mansoni only one thermolabile form of alkaline phosphatase was isolated which corraparded to twe primary isodynamic form in normal serum.
Studies were also done on the different molecular forms of human alkaline phosphatase. From live, small intestine, bone and placenta, originating from tissues considered as normal, and from Schistosoma mansoni itself.
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Recebido para publicação em 23.10.1980.
* A tese foi orientada pelo Professor Dr. Luiz Gonzaga de Azevedo Accioly , Chefe de La boratório de Enzimologia do Departamento de Bioquímica do Centro de Ciências Biológicas da UFPe
