Ernesto Hofer
Departamento de Microbiologia e Imunologia do Instituto Oswaldo Cruz C. P. 926. Guanabara
RESUMO
Foi pesquisada em 107 amostras de fezes procedentes de indivíduos com distúrbios intestinais a presença de Listeria monocytogenes.
Como processo de enriquecimento, as fezes foram semeadas em caldo triptose fosfatado e mantidas a 4oC durante 1 mês. Ao findar este prazo, foram os espécimes semeados em quatro meios seletivos: 1) Agar triptosado com 5 mcg/ml de Polimixina; 2) Agar triptosado com 50 cmg/ml de ácido nalidixico; 3) Meio de Ralovich e cols (Agar com 5% de soro normal de cavalo, acrescido de 50 mcg/ml de ácido nalidixico e 50 mcg/ml de tripaflavina (Bayer); 4) Uma modificação do meio de Ralovich (Agar triptosado, contendo 40 mcg/ml de ácido nalidixico, 50 mcg/ml de acetato de tálio, 25 mcg/ml de tripaflavina e 0,3% de extrato de levedura).
Para reconhecimento das colônias suspeitas nos diferentes meios, fez-se uso da técnica de Henry. A identificação primária se baseou na observação da motilidade e nas características morfotintoriais, senão a seguir, confirmada através das provas bioquímicas e sorológicas.
Três amostras de Listeria monocytogenes foram isoladas, caracterizando- se duas no sorotipo 4b e uma no tipo 1/2a.
ABSTRACT
The occurrence of Listeria in faeces was investigated in 107 samples of per sons with enteric disturbance. For enrichment, Tryptose Phosphate broth was used and incubated at 4°C for 4 weeks. Subsequent at the cold-enrichment, the cultures were streaked on plates of four selective media: Tryptose agar with 5 mcg/ml of polymyxin B; Tryptose agar with 50 mcg/ml of nalidixic acid; Trypaflavin-Nalidixic acid, described by Ralovich and a modification introduced in the last medium (Tryptose agar containing 40 mcg/ml of nalidixc acid, 50 mcg/ml of tallous acetate and 25 mcg/ml of trypaflavin). ldentification of suspected colonies was read under a scanning microscope using the oblique light technique according to Henry (14).
In three of these faecal specimens, Listeria was isolated: two strains belonged to serotype 4b against only one to type 1/2a.
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Recebido para publicação em 5-3-1974.
Apresentado na IV Conferência Internacional sobre os Impactos Globais da Microbiologia Aplicada (GIAM IV), 23-28 de Julho de 1973. São Paulo.
