Home » Volumes » Volume 8 March/April 1974 » Pesquisa sobre a ocorrência de histeria monocytogenes em fezes humanas

Pesquisa sobre a ocorrência de histeria monocytogenes em fezes humanas

Ernesto Hofer

Departamento de Microbiologia e Imunologia do Instituto Oswaldo Cruz C. P. 926. Guanabara

DOI: 10.1590/S0037-86821974000200008


RESUMO

Foi pesquisada em 107 amostras de fezes procedentes de indivíduos com distúrbios intestinais a presença de Listeria monocytogenes.
Como processo de enriquecimento, as fezes foram semeadas em caldo triptose fosfatado e mantidas a 4oC durante 1 mês. Ao findar este prazo, foram os espécimes semeados em quatro meios seletivos: 1) Agar triptosado com 5 mcg/ml de Polimixina; 2) Agar triptosado com 50 cmg/ml de ácido nalidixico; 3) Meio de Ralovich e cols (Agar com 5% de soro normal de cavalo, acrescido de 50 mcg/ml de ácido nalidixico e 50 mcg/ml de tripaflavina (Bayer); 4) Uma modificação do meio de Ralovich (Agar triptosado, contendo 40 mcg/ml de ácido nalidixico, 50 mcg/ml de acetato de tálio, 25 mcg/ml de tripaflavina e 0,3% de extrato de levedura).
Para reconhecimento das colônias suspeitas nos diferentes meios, fez-se uso da técnica de Henry. A identificação primária se baseou na observação da motilidade e nas características morfotintoriais, senão a seguir, confirmada através das provas bioquímicas e sorológicas.
Três amostras de Listeria monocytogenes foram isoladas, caracterizando- se duas no sorotipo 4b e uma no tipo 1/2a.


ABSTRACT

The occurrence of Listeria in faeces was investigated in 107 samples of per sons with enteric disturbance. For enrichment, Tryptose Phosphate broth was used and incubated at 4°C for 4 weeks. Subsequent at the cold-enrichment, the cultures were streaked on plates of four selective media: Tryptose agar with 5 mcg/ml of polymyxin B; Tryptose agar with 50 mcg/ml of nalidixic acid; Trypaflavin-Nalidixic acid, described by Ralovich and a modification introduced in the last medium (Tryptose agar containing 40 mcg/ml of nalidixc acid, 50 mcg/ml of tallous acetate and 25 mcg/ml of trypaflavin). ldentification of suspected colonies was read under a scanning microscope using the oblique light technique according to Henry (14).
In three of these faecal specimens, Listeria was isolated: two strains belonged to serotype 4b against only one to type 1/2a.


 

 

Texto completo disponível apenas em PDF.

Full text available only in PDF format.

 

 

REFERÊNCIAS BIBLIOGRÁFICAS

1. BEARNS, R.E. & GIRARD, K.F. – On the isolation of Listeria monocytogenes from biological specimens. Am. J. Med. Technol., 25: 120-126, 1959.         [ Links ]

2. BEERENS, H. & TAHON – CASTEL M.M. – Milieu à l’ácide nalidíxique pour l’isolement des streptocoques, D. pneumoniae. Listeria. Erysipelothrix. Ann. Inst. Pasteur, 111: 90-93, 1966.         [ Links ]

3. BLENDEN, D. C. & SZATALOWICZ, F.T. – Ecologic aspects of Listeriosis. J. Amer. Vet. Med. Ass., 151: 1761- 1766, 1967.         [ Links ]

4. BOCKEMÜHL, J., SEELIGER, H. P. R. & KATHKE, R. – Acridinfarbstoffe in Selektivährböden zur Isolierung von Listeria monocytogenes. Med. Microbiol. Immunol., 157: 84-95, 1971.         [ Links ]

5. BOJSEN-MOLLER, J. – Studies on the growth and isolation ol Listeria monocytogenes at low temperature. Second Symp. Listeric Infection (Ed. M.L. Gray). – Bozeman, Montana State College, pg 169-182, 1963.         [ Links ]

6. BOJSEN-MOLLER, J. – Growth of Listeria monocytogenes in mixed cultures and isolation from faeces after incubation 4°C. Proc. Third Internat. Symp. Listeriosis, Bilthoven, pg. 51-63, 1966.         [ Links ]

7. BOJSEN-MOLLER, J. & JESSEN, O. – Occurrence of Listeria monocytogenes in human faeces: Epidemiological and Pathogenic Aspects. Proc. Third Internat. Symp. Listeriosis, Bilthoven, pg. 415-421, 1966.         [ Links ]

8. BOJSEN-MOLLER, J. – Human Listeriosis . Diagnostic, Epidemiological and Clinical Studies. Acta Path. Microbioll. Scand., section B. suppl. 229, 157, pg., 1972.         [ Links ]

9. DONKER-VOET, J. – A serological study of some strains of Listeria monocytogenes isolated in Michigan. Am. J. Vet. Res., 20: 176-179, 1959.         [ Links ]

10. DONKER-VOET, J. – A serological study of some strans of Listeria monocytogenes. Proc. Third Internat. Symp. Listeriosis, Bilthoven, pg. 133-137, 1966.         [ Links ]

11. GRAY, M.L., STAFSETH, H.J. & THORP, F. – The use of sodium azide, potassium tellurite, and acetic acid in a selective medium for isolation of Listeria monocytogenes J. Bacteriol, 59: 443-444, 1950.         [ Links ]

12. GRAY, M.L. – A rapid method for the detection of colonies of Listeria monocytogenes. Zbl. Bakt. Abt. I Orig., 169: 373-379, 1957.         [ Links ]

13. GRAY, M.L. & KILLINGER, A.H. – Listeria monocytogenes and Listeric Infections. Bact. Rev. 30: 309-382, 1966.         [ Links ]

14. HENRY, B.S. – Dissociation in the genus Brucella. J. Inf. Dis., 52: 374- 402, 1933.         [ Links ]

15. HOFER, E. – Ensaios sobre a utilização de um meio com ácido nalidíxico e acetato de tálio para o isolamento de Listeria. Apresentado no III Congresso Brasileiro de Microbiologia, Belo Horizonte, Minas Gerais, 1871.         [ Links ]

16. JESSEN, O. & BOJSEN-MOLLER, J. – The frequency and the clinical types of human listeriosis. A four year study in Denmark. Second Symp. Literic Infections, Bozeman, Montana College State, pg. 255-262, 1963.         [ Links ]

17. JULIANE7LLE, L.A. – The function of Listerella in infection. Ann. Int. Med., 14: 608-620, 1940.         [ Links ]

18. KAMPELMACHER, E.H. & van NOORLE JANSEN, L.M. – Isolation of Listeria monocytogenes from faeces of clinically healthy humans and animais. Zbl. Bat. I Abt. Orig., 211: 353-350., 1969.         [ Links ]

19. KAMPELMACHER, E.H. & van NOORLE JANSEN, L.M. – Further studies on the isolation of L. monocytogenes in clinically healthy individuais. Zbl. Bakt. Hyg., I Abt. Orig. A. 221: 70-77, 1972.         [ Links ]

20. KAMPELMACHER, E.H. & van NOORLE JANSEN, L.M. – Isolierung von L. monocytogenes mittels Nalidixinsäuretrypaflavin. Zbl. Bakt Hyg., I. Abt. Orig. A, 221: 139-140, 1972.         [ Links ]

21. KRAMER, P. & JONES, D. – Media selective for Listeria monocytogenes. J. appl. Bact., 32: 381-394, 1969.         [ Links ]

22. LARSEN, E.H. – Listeria monocytogenes. Studies on Isolation Techniques and Epidemiology. Thesis. Carl Fr. Mortensen. Copenhagen. 256 pg. 1969.         [ Links ]

23. McBRIDE, M.E. & GIRARD, K.F. – A Selective method íor the isolation of Listeria monocytogenes from mixed bacterial populations. J. Lab. Clin. Med., 55: 153-157, 1960.         [ Links ]

24. MURRAY, E.G.D., WEBB, R.A. & SWANN, M.B.R. – A disease of rabbits characterized by a large mononuclear leucocytosis, caused by a hitherto undescribed bacillus Bacterium monocytogenes (n. s.). J. Path. Bact., 29: 407-439, 1926.         [ Links ]

25. ORTEL, S. – Ausscheidung von Listeria monocytogenes im Stühl fesunder Personen. Zbl. Bakt. I Abt. Orig., 217: 41-46, 1971.         [ Links ]

26. OSEBOLD, J.W. – Some aspects of the pathogenesis of listeriosis. Second Symp. Listeric Infection, Bozeman, Montana State College, pg. 109, 1963.         [ Links ]

27. RALOVICH, B., FORRAY; A., MÉRO, E. & MÁLOVICS, H. – Additional Data on Diagnosis and Epidemiology of L. monocytogenes. Zbl. Bakt. I Abt. Orig., 214: 231-235, 1970.         [ Links ]

28. RALOVICH, B., FORRAY, A., MÉRo, E., MÁLOVICS, H. & SZÁZADOS, I. – New selective medium for isolation of L. monocytogenes. Zbl. Bakt. I Abt. Orig., 216: 88-91, 1971.         [ Links ]

29. ROLLE, M. & MAYER, H. – Zur Pathogenese der Listeriose. Zbl. Bakt. 1 Abt. Orig., 166: 479-483, 1956.         [ Links ]

30. SEELIGER, H.P.R. – Listeriosis, 2 nd ed. Hafner Publishing Co. Inc., New York, 308 pg., 1961.         [ Links ]

31. SEELIGER, H.P.R., WINKHAUS, I., ANDRIES, L. & VIEBAHN, A. – Die Isolierung von Listeria monocytogenes aus Stuhl, Klarschlamn und Erdproben. Schweiz. Z. Path. Mikrobiol., 28: 590-601, 1965.         [ Links ]

2. WRAMBY, G.O. – Citado por Gray, M.L. & Killinger, A.H. in Listeria monocytogenes and Listeric Infections 1944.         [ Links ]

 

 

Recebido para publicação em 5-3-1974.

 

 

Apresentado na IV Conferência Internacional sobre os Impactos Globais da Microbiologia Aplicada (GIAM IV), 23-28 de Julho de 1973. São Paulo.