Wanderley de SouzaI; Noemia Grynberg II; F. Nery-GuimarãesII†
I Laboratório de Microscopia Eletrônica, Instituto de Biofísica U.F.R.J., Cidade Universitária, Rio de Janeiro - RJ II Laboratório de Protozoologia, Instituto Oswaldo Cruz, Rio de Janeiro - RJ
RESUMO
E feito um estudo da ultra-estrutura da forma epismastigota do Trypanosoma cruzi mantida em meio de cultivo acelular.
O núcleo das formas em divisão apresenta um aspecto homogêneo. Microtúbulos intranucleares são observados durante a divisão. No entanto, a membrana nuclear permanece íntegra.
O citoplasma apresenta-se com vacúolos de dimensões e aspectos variados.
Com o método do ácido periódico-tiosemicarbazida-proteinato de prata, polissacaríáeos e/ou glicoproteínas foram localizados na membrana celular e na membrana que delimita certos vacúolos citoplasmáticos.
SUMMARY
In this paper the fine structure of the epimastigotes forms of Trypanosoma cruzi maintained in an acellular culture medium, is described.
During division the nucleus shows a homogenous structure owing to the dispersion of its chromatin and nucleolar material. Microtubules similar to those of a mitotic spindle in metazoan cells appear, running frorn one pole to the other. During the whole process of division the nuclear membrane remains intact.
The cytoplam shows many vacuoles. With the cytochemical method of acid periodic-thiosemicarbazide-silver proteinate, a polysaccharide surface coat is observed on the whole surface membrane system of T. cruzi. A positive re- action is also observed in the membranes of some vacuoles in the cytoplasm of the parasites.
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REFERÊNCIAS BIBLIOGRÁFICAS
1. BAYNE, R. A., MUSE, K, E, & ROBERTS, J. F. – Regulation of oxidase enzyme systems in trypanosomes. Comp. Biochem. Physiol 30: 61-72, 1969. [ Links ]
2. BAYNE, R. A., MUSE, K. E. & ROBERTS, J. F Isolation of bodies containing the cyanide-insensitive glycerol-phophate oxidase of Trypanosoma equiperdum. Comp. Biochem. Physiol, 30: 1049-1054. 1969. [ Links ]
3. BRACK, C. – Electronem mikroskopische Untersuchungen zum Lebenszyklus von Trypanosoma cruzi. Acta Tropica 25: 289-356, 1968. [ Links ]
4. BRUN, R. – Ultrastruktur und Zyklus von Herpetomonas muscarum, Herpetomonas mirabilis und Crithidia luciliae in Chrysomia chloropyga. Acta Trop., 31: 219-290, 1974. [ Links ]
5. CAMARGO, E. P. – Growth and differentiation in Trypanosoma cruzi. I Origin of metacyclic trypanosomes in liquid media. Rev. Inst. Trop. São Paulo, 6: 93-100 1964. [ Links ]
6. DE SOUZA, W. & MEYER, H. – On the fine structure of the nucleus in Trypanosoma cruzi in tissue culture forms. Spindle fibers in the dividing nucleus. J. Protozool. 21: 48-52, 1974. [ Links ]
7. DE SOUZA, W. & MEYER, H. – An Electron Microscopical and Cytochemical study of the cell coat of Trypanosoma cruzi, in tissue cultures. Z. Parasitenk, 1975 “in press” [ Links ].
8. DE SOUZA. W. – Cytochemical detection of polysaccharides on the surface of Trypanosomatids. Trans. Roy. Soc. Trop. Med Hug, 1975 “in press” [ Links ].
9. DE SOUZA, W. – Associations membrane-microtubules chez Trypanosoma cruzi. J. Microscopie, “in press” [ Links ].
10. DE SOUZA, W., ROSSI, M. A., KITAJIMA, E. W., SANTOS, R. R. & ROITMAN, I. An Electron Microscopic of Leptomonas pessoai Submetido para publicação. [ Links ]
11. INOKI, S., OZEKI, Y. & KAMBARA, R. – Ultrastructural changes in the kinetoplast of Trypanosoma cruzi during transition of form in vitro. Biken J. 14: 37-50, 1971. [ Links ]
12. MARIA, T. A., TAFURI, W. & BRENER, Z. – The fine structure of different bloodstream forms of Trypanosoma cruzi. Ann. Trop. Med. Parasit 66: 423-431, 1972. [ Links ]
13. MEYER, H. & PORTER, K. R. – A study of Trypanosoma cruzi with the electron microscope. Parasitology, 44: 16-23. 1954. [ Links ]
14. MEYER, H., OLIVEIRA MUSACCHIO, M. & ANDRADE MENDONÇA, I. – Electron microscopic study of Trypanosoma cruzi in thin sections of infected tissue cultures and blood agar forms. Parasitology 48: 1-8, 1958. [ Links ]
15. MEYER, H. & QUEIROGA, L. T. – Submicroscopical aspects of Schizotrypanum cruzi in thin sections of tissue culture forms. J. Protozool. 7: 124-127, 1960. [ Links ]
16. MEYER, H. – The fine structure of the flagellum and kinetoplast chondriome of Trypanosoma (Schizotrypanum) cruzi in tissue culture. J. Protozool. 15s 614-621, 1978. [ Links ]
17. MEYER, H. – Further studies on the fine structure of the kinetoplast chondriome of Trypanosoma (Schizotrypanum) cruzi in thin sections of infected tissue culture. Rev. Inst. Trop. São Paulo 11: 48-56, 1979. [ Links ]
18. MEYER, H. & DE SOUZA, W. – On the fine structure of Trypanosoma cruzi in tissue culture of pigment epithelium from the chick embryo. Uftake of melanin granules y the parasite. J. Protozool. 20: 590-593, 1973. [ Links ]
19. MILDER, R. & DEANE, M. P. – The cytostome of Trypanosoma cruzi and T. conorrhini. J. Protozool. 16: 730- 737, 1969. [ Links ]
20. MUSE, K. E. & ROBERTS, J. F. – Microbodies in Crithidia fascicu lata, Protoplasma 78 : 343-348, 1973. [ Links ]
21. OZEKI, Y., SOOKSRI, V., ONO, T. & INOKI, S. – Studies on the ultrastructure of kinetoplasts of Trypanosoma cruzi and Trypanosoma gambiense by autoradiography and enzymatic digestion. Biken’s J. 14: 97-118, 1971. [ Links ]
22. PEREIRA DA SILVA, L. H. & NUSSENZWEIG, V. – Sobre uma cepa do Tripanosoma cruzi altamente virulenta para o camundongo branco. Folia Clin. Biol. São Paulo 20: 191-201, 1953. [ Links ]
23. RUDZINSKA, M. A. & TRAGER, W. – Intracellular phagotrophy by malaria parasites: an electron microscope study of Plasmodium lophurae. J. Protozool. 4: 190-199, 1957. [ Links ]
24. SANABRIA, A. – Ultrastructure of Trypanosoma cruzi in mouse myocardium. I. Trypanosome form. Exp. Parasit. 14: 81-91, 1963. [ Links ]
25. SANABRIA, A. – The Ultrastructure of Trypanosoma cruzi. II. Crithidial and Leishmanial forms. Exp. Parasit. 15: 125-137. 1964. [ Links ]
26. SANABRIA, A. – Ultrastructure of Trypanosoma cruzi in mouse brain. Exp. Parasit. 23: 379-391, 1969. [ Links ]
27. SANABRIA, A. – Nuevos estúdios acerca de la ultrastructure dei Trypanosoma cruzi en el miocardio del raton. Acta Cient. Venezolana 21: 107-118, 1970. [ Links ]
28. SCHULZ H. & MACCLURE. E. – Elektronenmikroskopische Untersuchungen des Trypcmosoma cruzi mit besonderer Berücksichtigung des periplasten und des Blepharoplasten. Z. Zellforsch. 55: 389-412, 1961. [ Links ]
29. STEIGER, R. F. – On the ultrastructure of Trypanosoma (Trypanozoon) brucei in the course of its life cycle and some related aspects. Acta Tropica 30: 64-168, 1973. [ Links ]
30. THIÉRY, J. P. – Mise en évidence des polysaccharides sur coupes fines en microscopie electronique. J. Microscopie electronique. J. Microscopie 6: 987-1018, 1967. [ Links ]
31. VICKERMAN, K & PRESTON, T. M. – Spindle microtubules in the dividing nuclei of trypancsomes. J. Cell Sci. 6: 365-383, 1970. [ Links ]
32. WERY, M. & DE GROODT-LASSEEL, M. – Ultrastructure de Trypanosoma cruzi en culture sur milieu semi-synthétique. Ann Soc. belge Méd. Trop. 46: 337-348, 1966 [ Links ]
Recebido para publicação em 21.5.1975.
† Lamentamos informar o falecimento do Prof. F. Nery-Guimarães em 15-4-1975. A sua amizade e a maneira com que se dedicava à investigação científica ficarão para sempre na memória de todos aqueles que com ele conviveram.
