Hermann G. Schatzmoryr
Professor-Adjunto de Microbiologia do Departamento de Ciências Biológicas da Fundação Ensino Especializado de Saúde Pública. Ex-Bolsista da Fundação Humboldt
RESUMO
Inibidores contra as amostras de poliovírus do tipo 1, Mahoney e CHAT, foram estudados no sistema de gel de hidróxido de alumínio. O inibidor comportou-se como uma macroglobulina do tipo 19 S (IgM), através filtração em gel (Sephadex G-200), partição em DEAE-Celulose e sedimentação por ultra-centrifugação em gradiente de sacarose. O inibidor foi ainda destruído através o tratamento com 2-Mercapto-Etanol.
O inibidor une-se à superfície do vírus na ausência de células. O complexo formado pelo vírus e o inibidor pode ser precipitado por um sôro preparado contra a globulina bovina; o mesmo complexo dissocia-se em idêntico valor de pH ao do complexo vírus-anticorpo 19 S.
A chamada infecciosidade residual (cêrca de 10%) que permanece após a neutralização de poliovírus com sôro bovino representa uma dissociação parcial do inibidor da partícula de vírus, quando em presença das células em cultura.
Foram obtidas amostras de vírus resistentes à ação específica de soros bovinos. Estas amostras permanecem sensíveis à ação de outros soros bovinos ativos, bem como à ação de anticorpos 7 S e 19 S.
Através experiências de adsorção de atividade inibidora, foi possível mostrar a existência de inibidores monoespecíficos, com diferentes especificidades contra as amostras CHAT e Mahoney, respectivamente.
ABSTRACT
Inhibitors in some bovine ser a, active against Poliovírus type 1, strains CHAT and Mahoney, have been examinated in Al (HO)- gel sistem. The inhibitor belongs to the 19-S macro-globulins as could be shown by Sephadex-200 gel filtration, fractionation through DEAE-Celulose and centrifugation in sucrose gradient.
Besides that the inhibitor could be destroyed through treatment with 2-Mercapto-Ethanol.
The inhibitor combines with the virus surface in absence of cells. The complex virus-inhibitor was precipitated by the use of anti-bovine-globulin serum; the complex virus-inhibitor dissociates by the same pH value as the complex virus-19 S antibody.
The author was able to show that by the neutralization of Poliovírus with bovine serum, in a cell system, the called “rest-infectiosity” (about 10%) represents a partial dissociation of virus particles which have been first bound to the inhibitor.
Resistent strains against specific bovine sera have been obtained. These strains, however, were still sensible to other bovine sera, as well as to 19-S and 7-S Polio antibodies. Through absorption experiments the presence of mono-specific activity of certain bovine sera, against the strains CHAT and Mahoney of Poliovírus has been shown.
Texto completo disponível apenas em PDF.
Full text available only in PDF format.
BIBLIOGRAFIA
1 – AGOL, V.I. and CHUMAKOVA, M. Y. – An agar polysaccliaride and marker of poliovírus Virology. 17: 221, 1962. [ Links ]
2 – ARMSTRONG, G. – Sucessful transfer of the Lansing strain of poliomyelitis virus from the cotton rat to the white mouse. Pub. Health Rep. 54: 2302, 1939. [ Links ]
3 – BARTELL, P. and KLEIN, M. – Neutralizing antibody to viruses of poliomyelitis in sera of domestic animais. Proc. Soc. Exper. Biol. Med. 90: 597, 1955. [ Links ]
4 – BENGSTSSON, S. and PHILIPSON, L. – Countercurrent distribution of polioviruses type 1. Virology. 20: 176, 1963. [ Links ]
5 – DULBECCO, R. and VOGT, M. – Plaque formation and isolation of pure lines with Poliomyelitis viruses. J. Exper. Med. 99: 167, 1954. 1959 [ Links ]
6 – DULBECCO, R. VOGT, M. and STRICKLAND, A.G.R. – A study of the basic aspects of neutralization of two animal viruses, Western equine encephalitis virus and poliomyelitis virus. Virology. 2: 162, 1956. [ Links ]
7 – FAHEY, J. L. and HOJtBETT, A.P. – Human gamma-globulin fractionation on anion exchange cellulose colurmns. J. Biol. Chem. 234: 2645, [ Links ]
8 – FLEXNER, S. and LEWIS, P.A. – Experimental poliomyelitis in monkeys. Eighthnote: further contributions to the subjects of immunization and serum therapy. J. Am. Med. Assoc. 55: 662, 1910. [ Links ]
9 – GARD, S. – Immune-inactivation of polioviruses Arch. ges. Virusíorsch. 7: 49, 1957. [ Links ]
10 – GERLOFF; R.O., HOYER, B.G. and 20 MAC LAREN, L.C. – Precipitation of radiolabeled poliovírus with specific antibody and antiglobulin J. Immunol. 89: 559, 1962. [ Links ]
11 – HAMMON, W. MC D., MACK, W.N. and REEVES, W.C. – The significancé of protection tests with the serum of man and other animais against the Lansing strain of poliomyelitis virus. J. Immunol. 57: 285, 1947. [ Links ]
12 – HAMPIL, B., MELNICH, S.L., WALLIS, G .BROWN, R. W., GRAYE, E.T. ADAMS, R.R. JR. – preparation of antiserum to enteroviruses in large animais. J. Immunol. 95: 895. 1965. [ Links ]
13 – HIRST, G.K. – Genetic recombination with New-Castle Disease vírus. Poliovírus and Influenza. Cold Spring Harbor Symp. Quant. Biol. 27: 303, 1962. [ Links ]
14 – KLEIN, M. – The signiíicance of human antiviral neutralizing substances in animal sera. Ann. N. Y. Acad. Sci. 70: 362, 1958. [ Links ]
15 – LEDINKO, N. – Genetic recombination with Poliovírus type 1. Studies of crosses between a normal horse serum-resistent mutant a íd several guanidine resistent mutants of the same strain. Virology. 20: 107, 1963. [ Links ]
16 – LEVINTOW, L. and DARNELL, J.E. – A simplified procedure for purification of large amounts of poliovírus: Characteristics and amino-acid analyses of type 1 poliovírus. J. Biol. Chem. 235: 70, 1960. [ Links ]
17 – MANDEL, B. – Reversibility of the reaction between poliovírus and neutralizing antibodies of rabbit origin. Virology. 14: 316, 1961. [ Links ]
18 – MC FERRAN – Poliovírus-neutralizing substances in cattle sera. J. Path. Bact. 83: 73, 1962. [ Links ]
19 – MC FERRAN, J.B. – A Substance in sera of man and animais neutralizing a bovine enterovirus. J. Path. Bact. 83: 83, 1962. [ Links ]
20 – PAGANO, J.S. and BõTTINGER, M. – Studies of the vaccine virus isolated during a trial with an attenuated poliovírus vaccine prepared in a human diploid cell strain, including use of a strain specific bovine serum inhibitor. Arch. ges. Virosforsch. 15: 19: 1964. [ Links ]
Tese de Doutoramento apresentada ao Instituto de Virologia da Universidade de Giessen, na República Federal Alemã, em dezembro de 1966.
